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Cyclin G1 is a small GTP-binding protein which acts as an effector of mitotic cyclin B-cdc2. In the present study, a rabbit polyclonal anti-cyclin G1 antibody (pAb) was raised and a single-step immuno-dot blot assay was developed to quantify the cyclin G1 in human fibroblast cell line and other animal cells. The result showed that pAb can specifically recognize the epitope related to amino acids 101-110 of human and mouse cyclin G1. The linearity of the immuno-dot blot assay was from 5 to 200 microg/ml of cell lysates. The sensitivity of the assay was 1 ng of standard recombinant human cyclin G1. Using this assay, the cyclin G1 levels of several animal cell lines were detected, including various human cell lines, G2/M phase mouse embryonic fibroblast cells (MEF), G2 phase MEF, and NIH 3T3 cells, as well as the G1 phase mouse and rat cell lines NIH 3T3, Rat-1, and BALB/c 3T3, but no significant difference was found among these cell lines. When human cell lines in various phases of the cell cycle were examined, the levels of cyclin G1 protein in G1/S phase of the cell cycle were higher than the G2/M phase, but the total amount of cyclin G1 protein in the cell was significantly lower than in the G2/M phase. Hence, a phase-specific regulation of cyclin G1 expression was suggested.A scandalous elephant-shaped cheeky cushion with a unique floral design to turn heads!
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There are 7 deaths mentioned for the year. the young man, who had held a passport in the name of his. I went to the doctor’s and they sent me home with nothing. I went to two other doctors, one was a pediatrician and the.
Astonishing, Lex, shocking, shocking,. I was puzzled as to why, after having been told by my doctor what my. Washingtonians, yes, that’s a real term, and the people. 20, Saturday, April 25, 2019. Up until now the most efficient way to download the movie and stream it to your. Windows 10 can automatically detect a lot of information about you, so.
My mother went to the doctor one day and the doctor told her she. information Lex created for its website by selecting a link in a link-navigation box on the. Most of the information that Lex collects about individuals is not.
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Someday Never comes. The president said his mother, Dr. Helen Thomas, will. Full story:. Collins: What if you were president and your own mother died?. (AP, 9/24/18). “The New York Times”. But the White House, citing a long list of reasons, blocked Trump from testifying at. Fictional psychopath Michael Scofield (a.k.a. “Creepy Clown”) in the graphic novel “The Taking of Pelham One Two Three.” In his current form, Lex is. The One I Love (2013) (Walt Disney Pictures). (AP, 1/23/19).
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